Dr. James Yates is a group leader at ITQB NOVA. His research focuses on the study and development of both biological and solid-state nanopores. His lab uses alpha-hemolysin as a nanotool for investigating the binding and properties of various small molecules or nanoparticles (analytes). The goal is to develop solid-state nanopores for diverse applications in defence, civil security, food safety, drug detection and drug testing, among others.

Abstract

“Adapting Solid-State Nanopore Platforms for the Green G Initiative”

Solid-state nanopores serve as a versatile platform for single-molecule sensing with applications in DNA sequencing, proteomics, molecular characterization, and polymer data storage. Solid-state nanopore devices consist of thin suspended dielectric films supported on silicon frames, with nanopores fabricated in the thin film region. The device is placed in a salt buffer solution and a potential difference is applied Precise current measurements (typically 100s of pA to 10s of nA) are made at sampling rates in the kHz range. Such experiments require precisely manufactured experimental platforms that allow for the nanopore chip to be accurately positioned and then sealed in place. In the Single Molecule Processes Laboratory, our expertise lies in the design and fabrication of such experimental platforms.

Here, we are proposing to transfer these experiences into building platforms for making current-based measurements in plant and fungal networks. In particular, our experiences of fabricating micro and nanoelectrode devices will allow us to monitor electrical signals in hyphae or across larger networks found in the environment. The aim with this talk is to promote discussion: I will present the capabilities that we possess and the types of devices that can be fabricated. The Green G community will then be asked to refine these ideas – to help design the hardware, sensors and analytics that will be needed to study plant-to-plant communication and how fungi contribute to these processes.